Yes. I did it for a long while. Don't let the tire ever get low on air, the tube type tire does not lock to a tubeless rim as well as a correct tubeless tire does, it can slip under hard braking to rip valve stem off if pressure is too low and the tire to rim lock does not lock as well. If you can air the tire up and at full pressure still see a clearance at the rim edge that a fingernail will hang on then the indicator of that less than ideal fit. A tubeless tire on tubeless rim has the tire go all the way tight to rim with no slight clearance there at all. You may have to install rim locks to absolutely stop the tire from slipping on the rim under hard braking. Then again, it may not slip at all. I had no locks in my case and no issues, but I've seen another that had to add the locks to stop trouble.